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KMID : 0545120150250030366
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 3 p.366 ~ p.374
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Ethanol Production from Glycerol Using Immobilized Pachysolen tannophilus During Microaerated Repeated-Batch Fermentor CulturePachysolen tannophilus, Glycerol, Ethanol production, Immobilization, Celite, Microaeration
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Cha Hye-Geun
Kim Yi-Ok
Choi Woon-Yong
Kang Do-Hyung
Lee Hyeon-Yong
Jung Kyung-Hwan
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Abstract
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Herein, we established a repeated-batch process for ethanol production from glycerol by immobilized Pachysolen tannophilus. The aim of this study was to develop a more practical and applicable ethanol production process for biofuel. In particular, using industrial-grade medium ingredients, the microaeration rate was optimized for maximization of the ethanol production, and the relevant metabolic parameters were then analyzed. The microaeration rate of 0.11 vvm, which is far lower than those occurring in a shaking flask culture, was found to be the optimal value for ethanol production from glycerol. In addition, it was found that, among those tested, Celite was a more appropriate carrier for the immobilization of P. tannophilus to induce production of ethanol from glycerol. Finally, through a repeated-batch culture, the ethanol yield (Ye/g) of 0.126 ± 0.017 g-ethanol/g-glycerol (n = 4) was obtained, and this value was remarkably comparable with a previous report. In the future, it is expected that the results of this study will be applied for the development of a more practical and profitable long-term ethanol production process, thanks to the industrial-grade medium preparation, simple immobilization method, and easy repeated-batch operation.
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KEYWORD
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Pachysolen tannophilus, glycerol, ethanol production, immobilization, Celite,
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